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KMID : 0606920170250030315
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Biomolecules & Therapeutics
2017 Volume.25 No. 3 p.315 ~ p.320
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Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells
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Yao Cheng-Wen
Kang Kyoung-Ah
Piao Mei Jing
Ryu Yea-Seong
Fernando Pattage Madushan Dilhara Jayatissa
Oh Min-Chang
Park Jeong-Eon
Shilnikova Kristina
Na Soo-Young
Jeong Seung-Uk
Boo Sun-Jin
Hyun Jin-Won
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Abstract
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We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.
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KEYWORD
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Autophagy, 5-Fluorouracil, SNUC5/5-FUR, Reactive oxygen species, Colon cancer
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